HBV was extracted using the Macherey Nagel Viral DNA kit. After that we went and measured the quantity of DNA by Qubit 4. the values were very low and some too low. Should an amplification be done before this step?
You can extract DNA after concentrating HBV using PEG
https://www.abcam.com/peg-virus-precipitation-kit-ab102538.html
However, Its really hard to get measurable DNA from Virus
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