There are many ELISA's optimized for Plasma/Serum both single and multiplex. You can also use flow based cytokine kits that are multiplex. The Luminex platform is also used often if you have access to a compatible machine and multiplexes for very large panels. Just confirm on the vendor site it will work for plasma/serum prior to purchasing.
Single Plex ELISA
https://www.rndsystems.com/products/human-il-2-quantikine-elisa-kit_d2050
https://www.biolegend.com/en-us/products/human-il-2-elisa-max-deluxe-2839
Flow:
https://www.bdbiosciences.com/documents/CBA_Brochure_Intl.pdf
https://www.biolegend.com/en-us/products/legendplex-human-cytokine-panel-2-13-plex-10639
Luminex
https://www.thermofisher.com/content/dam/LifeTech/global/promotions/global/images/aai-2015/aai-pdfs/CO123353-Luminex-brochure.PDF
As William Suslovic said, you could use single/multiple antigen ELISAs or multiplex bead based technologies. Single antigen ELISAs are easier to perform and all you need is a plate reader that can measure absorbance. Some of the multiplex ELISAs can also be done using a plate reader. Bead based techniques like CBA or Luminex would need access to specialized equipment like flow cytometers or luminex instrument.
Dear Dr Julie
Look Article Cytokine profile and lymphocyte subsets in type 2 diabetes
l hope help you
Good luck
Dear Azuka Patrick Okwuraiwe
May I suggest Ella by Simple Plex. An automated ELISA. Helps in avoiding operating factor that might affect the final measurement, faster results, and more economical if compared with ELISA kits.
https://www.proteinsimple.com/ella.html
Dear Azuka,
You can check Proteome Profiler Human XL Cytokine Array Kit. You can detects 105 human cytokines simultaneously in serum or plasma.
Good Luck
The most commonly used methods for cytokine quantification are the enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR).
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