I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of antigen. One antibody will be used as coating ab (Ab-1) and another as detecting antibody (Ab-2) and vice versa. I will also use an anti-rabbit HRPO conjugated secondary antibody to bind with detecting antibody.

Is there any chance that the secondary ab will bind with the coating ab also?

Please help.

More Prasanna Pal's questions See All
Similar questions and discussions