Mehri Hajiaghayi

3 Questions 5 Answers 0 Followers

Questions related from Mehri Hajiaghayi

Why do we have two fixed bands by using media in the western blot?

We are planning to check the level of phosphorylation of NF-KB in the CD4+ T cell population after activation with anti-CD3/CD28 and treatment for 15 minutes. However, I constantly see two fixed...

30 October 2023 1,542 1 View

Is this fine if we've seen 2 big bulbs near the lymphocyte population in PBMCs after 5-7 days cell culture?

Hi everyone, We wanted to do Intracellular cytokine staining on activated T cells and need to make sure about their gate in the flow-cytometer. We're using isolated human PBMCs and activating them...

10 August 2022 6,624 6 View

Can we use Tween20 as a solvent for Nebivolol?

Hi everyone, Due to poor water solubility of Nebivolol, I wonder if someone knows the possibility of dissolving it in Tween 20 for cell culture. Does it work or may it be toxic for PBMCs? Best...

13 February 2022 2,494 5 View