Researching and responding to a question requires that we first understand the context of the question posed. A sample of what? The answer depends on many things, but you have not provided any HPLC method or sample details needed to review your application. "HPLC" is an analytical technique, with many modes and possible methods, not a single method. Please provide the basic HPLC method details (i.e. column type, dimensions, mobile phase composition, detector settings, flow rate etc) PLUS information regarding what your sample is (name, properties etc) and what is "in" (matrix). *To save time, please try a keyword search on the web (i.e. GOOGLE) to find example HPLC methods for YOUR specific sample type. Have the info reviewed by a local professional chromatographer to see if the info can be applied to your application.
Hi Yousif Taha! Of course, you can purify it by HPLC! Just be aware of using normal phase HPLC (Hex/MeOH and silica gel ) instead of using RP-HPLC (H2O/AcN and C18/8)
Good luck! ;-)
Of course, you must be extremely careful about toxicity. I do not recommend using it, look for a solvent with similar polarity. It is carcinogenic
Generally no! Toluene is viscous (thus a high backpressure) and has a high UV spectra. However, Toluene as an extraction solvent will elute in the solvent front. But this means you will have to use a non-UV method (MS?) if Toluene is in the mobile phase or develop a non-Toluene mobile phase in which your molecule of interest is partially soluble. Check the internet for HPLC ideas /methods (ResearchGate.com, Phenomenex, Waters, Agilent...)
As mentioned earlier, there isn't enough information for us to advise you well. What column and solvents are you using? I will occasionally dissolve samples in toluene when running normal phase chromatography. It is a weaker solvent than ethyl acetate, and can help the injection.
For reverse phase, toluene would only work as an injection solvent if the mobile phase is mostly organic solvent because toluene isn't miscible with water. In this case, toluene is a stronger solvent and may affect the peak shape.
For reverse phase, toluene would only work as an injection solvent if the mobile phase is mostly organic solvent because toluene isn't miscible with water. In this case, toluene is a stronger solvent and may affect the peak shape
WhereasTo save time, please try a keyword search on the web (i.e. GOOGLE) to find example HPLC methods for YOUR specific sample type. Have the info reviewed by a local professional chromatography to see if the info can be applied to your application
Ca Dr. Gaurav Bhambri: Why is it necessary for you to plagiarize my previous July 12th response? - - - "To save time, please try a keyword search on the web (i.e. GOOGLE)..."
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