Can we use Thioflavin T (ThT) staining for recognizing inclusion bodies in cells for microscopy?

01 September 2013 1 6K Report

In literature, there are many imaging studies on inclusion bodies especially in neurodegenerative diseases like Parkinson's disease which was done by ThS. I would be pleased to know if it is possible to do this kind of fluorescent microscopy with ThT?

David Gnutt

One problem you might encounter using ThT is unspecific binding of ThT to the cells. A study that used ThT in cell culture already in the context of amyloid fibril formation: doi 10.1074/jbc.M511174200

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