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Questions related from Amir T. Marvian
I was wondering if it is normal for neurons to immigrate into the edges of wells during differentiation. Are there any means to obtain a more homogenous distribution of cells in the well...
18 March 2021 6,375 6 View
We have four fluorescently labeled endocytic markers including human serum albumin (HSA-Atto488) labeled in house, human transferrin (hTf-Alexa488) from ''Biozol Diagnostica'', Lactosylceramide...
01 February 2020 7,146 0 View
I am doing cation exchange chromatography by using HiTrap-SP-HP column, and the buffer A is 20 mM Tris, 50 mM NaCl, 1 mM MgSO4, 1 mm DTT. Since the FPLC machine is old, after loading the protein,...
27 August 2018 7,262 9 View
We know that in vivo studies on mouse models of Parkinson's disease revealed high aggressive potential of misfolded protein to propagate in brain tissue and cause degeneration (Brundin et al.)....
12 June 2014 3,474 13 View
What do you think, is the drawing error-bar in excel only based on standard deviation or should we divide it by the square root of iterations?
25 November 2013 5,733 3 View
In literature, there are many imaging studies on inclusion bodies especially in neurodegenerative diseases like Parkinson's disease which was done by ThS. I would be pleased to know if it is...
01 September 2013 6,304 1 View
It seems that endocytosis is the most important factor for expansion of a-syn in brain tissue. Additionally, it seems that knock-out mice have no problem in their essential functions of the brain,...
26 June 2013 4,660 2 View
We have a DNA fragmentation ELISA kit from Roche. The protocol suggested NaOH treatment for 30 min as a positive control for DNA fragmentation. We did not get significant result, so I become...
27 May 2013 3,380 4 View
In fact, I want to measure intracellular Ca++ in a cell line but because of some financial problems we need a cheaper indicator than common fluorescent indicators.
13 March 2013 3,454 3 View
In my cloning project, I have to double digest a plasmid and then extract the big fragment from a agarose gel to use it for ligation process. But for finding the place of digestion product on the...
04 March 2013 6,119 10 View
I have a problem distinguishing my cells. The cells are treated, then stained with the Annexin-PI kit, and after that tested by flow cytometry. In the kit's manual, it is mentioned that these...
19 February 2013 6,225 58 View
Nowadays, famous companies like Merck and Thermo provide different ladders for native-PAGE analysis. However, basic biochemistry tells us that the movement of proteins on the native-PAGE is not...
01 January 1970 10,067 5 View
