Can we use the same house keeping gene primers for human and mouse samples?

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What is the maximum value of Divesity Gian in MIMO? For two antenna system its 10dB. As no. of antennna increse diversity gain increase or decrease?

Somewhere on the internet, it's reported the maximum diversity gain limit theoretically can be achieved is 10dB. Some papers said it increases with more antennae and some said it will decrease.

09 June 2024 5,121 2 View

Fully funded phd ?

any fully funded phd position in wildlife ?

25 May 2024 7,976 2 View

Please, anyone can get me this following book?

Surface Plasmon Resonance Imaging Basic Theory and Practical Methodology

16 May 2024 3,566 0 View

Access to document containing CPCB calibration curve and uncertainty for PM2.5, SO2, O3, and NO2 monitors?

I am wondering if anyone has come across calibration curve and uncertainty for PM2.5, SO2, O3, and NO2 monitors maintained and operated by the CPCB? Please share link herein for the same if you...

25 April 2024 7,164 5 View

How to model or program a cascade refrigeration system using natural refrigerants on MATLAB ?

What are different ways of modelling and simulation of cascade refrigeration cycleusing natural refrigerants on MATLAB without using simscape ? How to write program for it and connect MATLAB...

02 April 2024 487 2 View

Classical text is important or research journals are important?

because people are citing/talking less about Classical texts discovered by Rishi/sages. Rishi has the broad scientific knowledge as per their practical and theoretical practices.

30 March 2024 2,013 0 View

Lithium ion battery Equivalent circuit parameter estimation?

I tried parameterizing an ECM model with an EIS experimental data set using Matlab lsqcurvefit solver. The resulting EIS is in an acceptable range if you see the attached figures. But my doubt...

27 March 2024 4,292 0 View

What is the refractive index for antimone and how we find this?

optical property, particularly for 1550nm

26 March 2024 1,107 3 View

How to interpret the LCMS data with using peak values?

How to evaluate the compounds with usage of peaks and their respective values in LCMS - TOF instrumentation? Is there any web portals for the data interpretation? I Have got Both LC (2 Files) MS...

13 March 2024 7,940 1 View

EIS behaviour for different charge rates for Lithium ion battery DFN model ?

Hello Everyone, I am obtaining the EIS from the DFN model and the EIS profile is shifting to the right side when we increase the charge C rate from 0.3 to 1C or 3C (refer to the figure below)....

05 January 2024 8,808 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View