Can we use the same house keeping gene primers for human and mouse samples?

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How to do buckling analysis in ansys apdl for laminated plate with PZT 5H attached?

I am doing buckling analysis of laminated plate surface bounded with two piezoelectric PZT 5H and axial compressive load is applied. I am getting an error, No ANEL table exists for material 2....

16 February 2021 510 1 View

What is the difference between a shared aperture antenna and a dual band antenna?

Can the dual-band antenna be called as a shared aperture antenna? Difference between shared aperture antennas and dual-band antennas?

30 December 2020 7,118 4 View

Which furnace or method are suitable for direct melting of the powder ?

My question is very specific about melting of pure reduced Iron Fe in powder form

29 December 2020 4,352 3 View

How to make a standard curve for qpcr?

Hi there, I am working on airborne microbes. I want to know the concentration of bacteria in my sample before I go for NGS. Since it is an environmental sample it might have a lot of mixed...

17 November 2020 314 3 View

I have one question related to cross correlation and auto correlation in neural network training?

Autocorrelation value is 0.3 and input cross-correlation error value is 3.Are the obtained results after training ANN is correct or wrong?

04 October 2020 9,686 2 View

Poly ICLC or Poly IC structure for docking ?

I want to do docking of Poly ICLC with protein. But, unfortunately there is no crystal structure for Poly ICLC or poly IC. Poly ICLC is a complex of Poly IC, poly lysine, and...

24 September 2020 6,107 1 View

What is the best way to preserve fungal RNA in shipping (1 - 2 days)?

The sample (fungal RNA) extracted with RNeasy Kit is supposed to be sent for sequencing.

15 September 2020 7,128 3 View

How to cluster the ORF sequences?

Hi, I have found the ORF region of my nucleotide sequence which was obtained from Sanger sequencing using 16s universal primer. I have to cluster them before using them in my model. I am using...

13 August 2020 1,079 4 View

How to measure the T1 and T2 value from a MR image ?

I have an MR Dicom file having values of repetition time, echo time, Inversion time, flip angle, etc. I want to calculate T1 and T2 from this Dicom file. Is there any way to calculate this from...

12 March 2020 7,598 12 View

I want to simulate the rcc frame in a software, can anyone please suggest me the best option for research work?

Wanted to compare the behaviour between ductile and non ductile detailed RCC frame specimen

04 February 2020 270 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View