Prashant Akila

3 Questions 3 Answers 0 Followers

Questions related from Prashant Akila

Can we use the same house keeping gene primers for human and mouse samples?

We have primers for human GAPDH gene. Can I use these primers when I perform real time PCR with mouse samples. Kindly please advice...

24 June 2015 4,983 2 View

Can fluoroscent antibodies from two different companies be used in the same reaction mixture for flow cytometric analysis?

I would like to check for CD133+/CD44+ population in colon cancer. I am planning to buy CD133-PE from miltenyi biotech and CD44-FITC from biogems. Kindly advice if I can go ahead with the purchase.

29 April 2015 7,114 6 View

What are the different ways to check if our primer pair is specific to a certain species of bacteria?

We have designed primers for specific bacterial species. The PCR is showing good results by amplifying only the specific bacteria with no amplification with other bacteria. How can I further...

01 January 1970 1,156 10 View