we planned to study about metallothionein gene expression study using RT -PCR . Is it Ok to use post mortem blood sample for gene expression analysis?
Yes you can use it
Hi, We're having a bit of a problem while trying to find progenitor populations in bone marrow. The protocol for CyTOF includes staining the cells, fixation, methanol perm for 15min, intracellular...
15 October 2020 997 3 View
I have planned to do cell based gene expression (human) study using disease suspected water sample. For that, I planned to use human cell lines. Still I don't have plan regarding water sample, how...
08 March 2018 3,837 3 View
We planned to do gene expression study using disease suspected water sample.
10 September 2017 4,930 8 View
I have done my soil washing using EDTA and got result at pH 8 gives more removal of Cr.What is the reason for base pH?usually removes for acid pH but for Cr gave more efficiency at pH 8..
14 June 2016 1,425 4 View
please tell any IS standards for permissible limit of heavy metals in soil?
06 May 2016 9,681 18 View
I want to maintain pH in base but also it should be in the nature of acid. My solution to test in ICP-AES for heavy metals is EDTA washed soil solution which is base nature. To keep in ICP, it...
09 April 2016 2,160 9 View
With condition one plant per pot and 30days growth period to ensure uniform growth.
05 January 2016 1,360 4 View
I would like to calculate the doubling time of some strains growing in phenol. Can anyone help me?
11 April 2012 4,963 2 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View