we planned to study about metallothionein gene expression study using RT -PCR . Is it Ok to use post mortem blood sample for gene expression analysis?
Yes you can use it
I performed FRAP on a lipid membrane and got the fluorescent intensity curves over time. I need to calculate the diffusion coefficient of the fluorescently labeled lipid. Can anyone suggest a...
07 July 2023 5,822 1 View
Is it good or bad for our well being?
17 May 2023 9,331 1 View
Chromatography techniques rarely follow linear processes. Two-level factorial designs cannot evaluate nonlinearity, and thus assume linearity with only two points. This defect leads to false...
10 November 2021 7,897 0 View
Hello! I have a gene expression dataset that underwent several transformations, after which I ran statistical tests. Every group in the data as well as every gene underwent the same linear...
19 October 2020 483 0 View
Hi, We're having a bit of a problem while trying to find progenitor populations in bone marrow. The protocol for CyTOF includes staining the cells, fixation, methanol perm for 15min, intracellular...
15 October 2020 1,148 3 View
I have planned to do cell based gene expression (human) study using disease suspected water sample. For that, I planned to use human cell lines. Still I don't have plan regarding water sample, how...
08 March 2018 3,965 3 View
If I filter the water samples using 0.45-micron filters and acidified (pH
02 February 2018 3,969 4 View
We are working in the water and waste water treatment.I need details about anoxic tank which is provided in Sewage Treatment plant. How much the removal efficiency of parameters?(BOB,COD etc.)...
04 January 2018 2,963 2 View
We planed to analysis of heavy metal,fluoride ,essential metals from disease suspected water. Do we need to follow microwave digestion ? Advantage?
08 October 2017 8,641 3 View
I want to assess the level of toxic metals, essential metals and ions especially Na,K,F from disease suspected water sample using ICP-MS. Is there any specific percentage for conHNO3 for storage...
11 September 2017 2,406 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View