Can we use high melting point agarose gel instead of low melting point gel in lower concentration for Comet assay?

14 June 2021 1 3K Report

we are designing experiment for genotoxic study and we don't have low melting point agarose gel.

Usually the high melting point gel is avoided because an increase temperature may cause damage to the nucleic materials and create false positives.

I suggest reading the responses to an older question asked on this site.

November 26, 2014

Neethu Kannan B, of the Siddha Regional Research Institute, asked :

Please suggest the relevance of using Normal Melting Point Agar and Low Melting Point Agar while doing comet assay?

at this link…

https://www.researchgate.net/post/Please_suggest_the_relevance_of_using_Normal_Melting_Point_Agar_and_Low_Melting_Point_Agar_while_doing_comet_assay

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