Dear All,

I am trying to establish a screening method for glutamate receptor antagonist (mGluR5) for preliminary screening.

Most of literature suggest use of primary neuronal culture for the same.

I read a paper which suggest the use of differentiated IMR-32 cells for the same assay. Although I tried to reproduce the same but failed to get any exocitotoxicity with Glutamate at 250 and 500 uM when exposed for 24 hrs in cell culture incubation conditions.

The cells were differentiated for 5-6 days with Retinoic acid.

L-Glutamic acid from Sigma was used to induce exocitotoxicity.

Link for the paper which I followed has been provided below.

Article Water Extract from the Leaves of Withania somnifera Protect ...

Please share your opinion over the same.

Regards

Shubham