What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 449 1 View

how to export a phylogenetic tree into word document using MEGA X?

I have built a tree of my interest but MEGA X displays an error when saving the image as PDF file! I was wondering how can i save/ copy the tree into a word file. Thanks all

09 October 2019 4,045 5 View

DO you recommend GC-MS/EI or CI?

I am gonna do some analyses / detection on plant secondary metabolites (Terpenes and mostly volatile ones), Was wondering which method might be more beneficial- GC-MS-EI or CI? In most recent...

09 October 2019 2,384 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

08 September 2019 4,083 3 View

Do I need to carbonize the EM formvar grids to visulize virus like particles?

Dear researchers, I am going to fix my virus like particle sample using negative staining. However, the grids i have are only formvar without carbon coated. Do you have any idea what difference it...

10 November 2018 5,842 7 View

Best method to resuspend and anneal oligose for downstream ligation?

Dear Researchers, I have ordered oligose which should be annealed together for cloning/ ligation experiment. They are 66 in length and stickiy ends using two different restriction enzymes. The MW...

06 July 2018 9,829 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

04 May 2018 1,635 2 View

Why my SDS PAGE get stuck in the middle of the gel and the last lanes of the ladder are not seperating properly?

Dear All, I recently have a problem with my SDS-PAGE running. After staining with Brilliant blue and de-staining, I see that the proteins have not been run until the end of the gel. I increased...

04 May 2018 4,267 13 View

Recommending a protocol for VLPs isolation from e.coli?

Hi all, Iam expresing a virus capsid protein in e.coli. I need an optimised protocol for isolation virus like particles from e.coli cells. Using ultarcentrifugation I have a protocol but usually...

04 May 2018 8,178 2 View

Does anyone know of a plant based-vaccine against HAV?

Hi all, I am looking for some papers/ publications where a Hepatitis A vaccine has been tried or made in any plant. To my knowledge, There are not that much study on plant based vaccine against...

01 February 2018 2,556 1 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View