If the binding stoichiometry is 1 and no additional effects are present (see https://www.researchgate.net/post/What_factors_should_I_consider_when_doing_a_reverse_titration_on_Isothermal_titration_calorimetry_to_avoid_a_low_solubility_ligand), the same binding isotherm should be observed in the direct and the reverse titration.