Kriti Shukla

4 Questions 1 Answers 0 Followers

Questions related from Kriti Shukla

Does anyone have experience with Proximity Ligand Assay?

I found a few kits by Sigma-Aldrich and want to make sure I am getting the right one for my experiment. Which kit should I be getting to study co-localization of two proteins in sub-cellular...

09 August 2022 143 1 View

Why is my protein sticking to G25 microspin column?

I am using cytiva microspin G25 columns to get rid of excess unicorporated ATP from my protein sample. The protein is 28kDa. The issue that I am facing is that after following the Cytiva protocol...

15 June 2021 2,757 4 View

Can we switch the cell and syringe content in ITC and expect same binding profile?

I want to put the ligand in the cell and protein in syringe.

04 December 2020 2,036 1 View

Can we use ITC to study protein- phosphate ion binding?

I need to test if my protein (100 amino acid residues) binds to phosphate ion.

04 April 2020 8,926 0 View