Can we store pierce glutathione agarose beads in 50 mM tris, 150 mM NaCl,pH 8.o ?

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Does there exist any empirical evidence of government expenditure financed by money collected through lobbying?

Basically, i am trying to understand from the literature whether lobbying can also considered an alternative source of financing fiscal deficit.

26 July 2024 8,692 5 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

How surfactant concentration affects the viscosity of nanofluids? Does viscosity increase with the increase of surfactant concentration?

In nanofluids, surfactants play a great role in stability. What is the impact of surfactant in viscosity in nanofluids? If surfactant concentration increases, then viscosity will increase/decrease...

24 June 2024 6,288 4 View

RNA isolation from plant roots infected with both bacteria and fungi. How to isolate RNA from all of them simultaneously?

I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...

09 June 2024 9,876 0 View

How to plot in Simu5G on Omnet++?

Dear network I need to plot instantaneous and mean End-to-end delay in D2D and eNode-based topologies? How to generate/visualise the accurate vec file data? I appreciate your help

14 May 2024 2,897 1 View

How can I find capacity of Li-Ion coin cell (CR2023) battery ?

if I want to run my battery cycling test in 1 C rate ,but then I need to know the capacity of my material used in the coin cell (i.e. if my capacity is 20mAh then I will put a constant current of...

05 May 2024 1,364 1 View

Boundary condition settings for Antenna simulation software CST or HFSS ?

I always have problems setting boundary conditions (Open, add space, Et=0, Ht =0, etc.) in CST Studio Antenna Simulation Software. Please find the attached figure. I designed all the antennas...

25 April 2024 8,761 1 View

I need ICCD / JCPDS data of LFP (LiFePO4) . If someone have that please forward it ([email protected]). How can i download that ?

I need ICCD / JCPDS data of LFP (LiFePO4) . If someone have that please forward it ([email protected]). How can i download that ? I need that to plot my XRD. what are the characteristic...

11 March 2024 3,872 1 View

Are there any suggestions or insights you can provide to help address this convergence failure in my optimization process?

I'm currently experiencing convergence failure while attempting to optimize a dimeric liquid crystal molecule under the influence of an electric field. The optimization code I'm using is...

10 March 2024 4,737 0 View

Does the dipole moment of a molecule correlate with its hyperpolarizability and polarizability?

implying that a molecule with a lower dipole moment exhibits lower hyperpolarizability and polarizability compared to one with a higher dipole moment?

07 March 2024 572 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View