Can we store blood samplesfor one day in normal EDTA vials for micro-RNA isolation?

MiRNA qpcr problem?

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Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

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What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

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Which miRNA arm is a right target for analysis, 3p or 5p?

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Is negative controls necessary for western blotting?

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Can I predict miRNA by knowing the mRNA target?

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What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

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Abderrahmane Bengrine

Dear Saliha,

Circulating miRNA is presumably not naked miRNA, which would be degraded within seconds due to the high levels of nucleases in blood. Several reports have demonstrated that stability results from the formation of complexes between circulating miRNA and specific proteins or been protected in the exosomes or vesicles.

With this I think that keeping the blood for one day before preparing mirRNA will be ok.

I hope this help

Mohammed Alhomidi

PLoS One. 2015 Nov 23;10(11):e0143321. doi: 10.1371/journal.pone.0143321. eCollection 2015.

Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance.

Leidinger P1, Backes C2, Rheinheimer S1, Keller A2, Meese E1

Saliha Rizvi

Assalamun alaikum Sir,

Thanku so much for your valuable answer. It solved my problem. :)