Can we stain fission yeast using Calcein-AM?

07 July 2016 0 2K Report

Can we "live stain" S. Pombe using Calcein-AM/Hoeschst (plasma membrane and cell wall as well as the septum and nucleus)?

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Science method

More Poleni Paul-Emile's questions See All

What would be the best way to create an ethnographic database in order to facilitate comparative work ?

I am seaching for a software that would enable me to create a database constitued of PDF files of older ethnographies of north american indigenous peoples. Here I'm thinking of ethnographies like...

19 January 2021 8,714 5 View

Aquamax DW4 Plate Washer Software?

Our lab recently inherited an Aquamax DW4 plate washer. This device is no longer supported/serviced by Molecular Devices (manufacturer). It did not come with the CD-ROM and we are wondering if...

10 June 2020 8,650 0 View

What is the best way to do QC with plink ?

This post is going to be a little long, so I apologize in advance for bothering you. I learned that quality control is super important so I want to make sure that I do this right. I have several...

18 May 2020 6,670 1 View

Can I add fresh DTT and PMSF to previous buffers?

I have a set of buffers (Tris, imidazole, phosphate) for NI-NTA protein purification. I prepared them about three weeks ago and they contain DTT and PMSF. Can I add fresh DTT and PMSF to the...

03 July 2019 2,670 3 View

Lipid abundances to enzyme pathways?

Imagine we had two subject groups: controls and patients with a certain condition, altering lipidomes of their blood. Then we obtained abundances of each molecular species for both groups and...

05 February 2018 8,386 1 View

Relation between film density / texturation / bandgap ?

Dear all, I deposited CrN film using different conditions. I observed a strong texturation along (111) direction (already reported in numerous publication), but a large variation of the films...

03 January 2018 5,236 3 View

Hot papers in soil science or soil polution ?

Hello everyone, I want to know how can i be updated on the hot papers/articles in soil science, soil pollution,... any help i will appreciate Thanks a lot for your replies.

24 August 2017 5,601 10 View

Are you or other searchers interested to extend the same study on Pleurotus spp of Madagscar ?

In Madagascar island, 6 unknown eaten pleurotoid occur in native humide forests or plants material wastes.

16 May 2017 1,240 2 View

Does tissue lysis by sonication affect the RNA integrity?

I am extracting RNA from FFPE tissue (Phenol Chloroform method). I am not being able to achieve complete homogenization with the tissue homogenizer. I was thinking about using sonication. Do you...

17 December 2016 254 1 View

Does Qiazol enables the extraction of proteins and DNA from the same sample along with RNA extraction?

I want to extract proteins and RNA from tissue samples. TRIzol reagent is not available in our labs. We have Qiazol instead? Does it do the same job? Are these products comparable in efficiency?...

13 October 2016 778 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View