Can we "live stain" S. Pombe using Calcein-AM/Hoeschst (plasma membrane and cell wall as well as the septum and nucleus)?
I am seaching for a software that would enable me to create a database constitued of PDF files of older ethnographies of north american indigenous peoples. Here I'm thinking of ethnographies like...
19 January 2021 8,522 5 View
I have a set of buffers (Tris, imidazole, phosphate) for NI-NTA protein purification. I prepared them about three weeks ago and they contain DTT and PMSF. Can I add fresh DTT and PMSF to the...
03 July 2019 2,480 3 View
24 August 2017 5,462 10 View
I want to extract proteins and RNA from tissue samples. TRIzol reagent is not available in our labs. We have Qiazol instead? Does it do the same job? Are these products comparable in efficiency?...
13 October 2016 619 3 View
I extracted DNA using extraction column. It appeared that there was a lot of cell debris in the extract. Lot of impurities appeared in the nanodrop specking. How can I remove the cellular debris...
09 July 2015 755 7 View
22 June 2015 9,110 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View