Can we reuse the silica DNA purification columns?

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Are diatom inclusions found in shellfish shells?

Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...

12 July 2024 159 2 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

How to produce homogeneous reference gene bands in Western Blot?

Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...

26 June 2024 5,913 3 View

Do you think undergraduate teaching via online platforms like zoom is sufficient to teach theory parts of Science subjects?

When I analyzed grades of undergraduates, some course taught via online platforms, their grades are lower than the physical lectures.

11 May 2024 7,475 4 View

Why Agroforestry not popular among farmers?

Agroforestry is considered and identified as a good land management practice. But most of the farmers prefer monoculture or mixed cropping system, But not agroforestry provide lots of tangible and...

09 May 2024 2,376 2 View

Which is the correct keyword for NLO calculations?

Hello, I hope this message finds you well. I have just started to learn gaussian software. While running NLO calculations I used the keyword: #p b3lyp/6-31g(d,p) geom=connectivity gfinput...

08 May 2024 6,756 2 View

Is it necessary to incorporate deformation energy and entropy corrections when calculating MMPBSA binding free energy in Protein-ligand complexes?

The inclusion of deformation energy and entropy corrections in MMPBSA binding free energy calculations is common in studies involving DNA-ligand complexes. However, in the literature, these terms...

25 April 2024 7,286 0 View

How to do Thrips Egg staining?

In order to view/ find the laid thrip eggs is there any methods is available, if yes, suggest me the methods.

23 April 2024 7,873 0 View

Would like to learn about Auguste Levasseur, Lafayette's friend and author of the book on their visit to the United States in 1824-25?

The Marquis de Lafayette is "returning" to America again this fall. After serving in the American and French Revolutions, he returned to America in 1824-1825 and toured some 25 states. This...

19 April 2024 4,979 0 View

Which software can be used for opening tfh files for analysing the results?

I have got measurement results from China. But they have given me the results in tfh file format. Can anyone suggest a software to open the file and analyse the results?

14 April 2024 789 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View