When reporting mediation in a manuscript being submitted for publication, what have you found to be the easiest way to create your path diagram?

I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.

11 January 2021 3,585 1 View

What method is more useful for prepare metal/ metal oxide Nanocomposites?

If possible any easy preparation method

31 December 2020 1,662 3 View

Advice on the culturing of EL4 lymphoma cell line?

Hi all, this is my first-time culturing lymphoma cell line. I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the...

04 November 2020 932 3 View

How to find a partner university for an Academic conference?

I am organizing a conference for the first time and I would like to partner with an University for the conference. What are the things that I should do to invite/request a university. Whom should...

04 October 2020 7,530 3 View

How does RNA mini kit get rid of DNA without using additional DNase treatment?

Hello everyone. I am an undergraduate student majoring in cardio-thoracic technology. I'm now working on my Bachelor's thesis that i use real time PCR (gene expression study between drug-treated...

11 August 2020 4,594 9 View

Can any one help me to perform EWAS ?

We have conducted an experiment on epigenetic allele identification and quantification for some quantitative trait. we are trying to map epigenes using epigenome association mapping (EWAS)....

10 August 2020 4,170 3 View

Application of machine learning in agriculture research?

Advances in data science throwing its impact on agriculture research data analysis. looking to understand applications of machine learning concepts in agriculture research. Thanks

29 June 2020 6,959 6 View

Behaviour, inducing stress in terrestrial invertebrates?

Hi, I found a number of behavioural papers studying anxiety behaviour following stress. Stress was induced by exposure to predators, agonistic interaction or electric shocks. But these are almost...

24 June 2020 309 1 View

How to find open access journals with no publication fees to publish a work?

How can a student be able to find open access journals with no publication fees to publish their work? A step by step procedure will be most appreciated.

04 May 2020 6,700 3 View

Is there any correlation between the impact factor of scientific journals and the average rate and time for rejection?

Is there any correlation between the impact factor of scientific journals and the average rate and time for rejection. Many high impact journals reject submissions even before peer review.

04 May 2020 262 14 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View