Are there any commercial CRISPR/Cas9 vectors suitable for potato transformation via Agrobacterium?

04 May 2016 2,927 3 View

After gene knockout, will incorporation of non-target tissue mRNA make significant error in measuring relative expression level against the wild type?

An SLR1 promoter is a tissue-specific promoter for exocyst genes which is strongly expressed only in stigma and not in the pollen or style. Suppose that we have an RNA knockout gene construct as...

31 December 2015 8,536 0 View

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies?

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies? Or are both of these TALEN pairs designed to be...

31 December 2015 258 2 View

Do tetraploid/ tetrasomic potatoes suffer from inbreeding depression as frequently as do the diploids during the conventional plant breeding?

31 December 2015 1,645 1 View

Can we say that the gene is probably regulated by the sugars if their promoters contain conserved cis-elements like other sugar-regulated genes?

11 December 2015 5,733 4 View

Can you please list some ways of creating variations in plants?

11 December 2015 5,404 3 View

Should I go for sequencing?

I transformed XL1B cells by cloning 16S rDNA gene of a thermophile strain to pTZ57R/T vector. The cloned plasmid vector isolated from white recombinant colonies were quite good in the gel, and the...

10 November 2015 2,418 5 View

Growing Agrobacterium at 28-30 in liquid culture overnight

Many papers and standard lab protocols describe growing Agrobacterium at 28 degrees. However, few papers also describe doing the same at 29 degrees, and some protocols give a freedom of 28-30...

01 January 1970 6,294 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View