Sarbesh Das Dangol

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Questions related from Sarbesh Das Dangol

Can we readily expect homozygous knockouts in the T0 plants in CRISPR knockout event of tomato plants?

Can we readily expect homozygous knockouts in the T0 plants in CRISPR knockout event of tomato plants? Or should we wait until the T1 plants after the selfing event?

09 September 2017 4,441 4 View

Are there any commercial CRISPR/Cas9 vectors suitable for potato transformation via Agrobacterium?

Can you please suggest me some commercial CRISPR/Cas9 vectors suitable for potato transformation via Agrobacterium?

05 May 2016 3,008 3 View

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies?

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies? Or are both of these TALEN pairs designed to be...

01 January 2016 331 2 View

Do tetraploid/ tetrasomic potatoes suffer from inbreeding depression as frequently as do the diploids during the conventional plant breeding?

Do tetraploid potatoes suffer from inbreeding depression as frequently as do the diploid potatoes during the conventional plant breeding? Please explain. Also, can you please elaborate on...

01 January 2016 1,717 1 View

After gene knockout, will incorporation of non-target tissue mRNA make significant error in measuring relative expression level against the wild type?

An SLR1 promoter is a tissue-specific promoter for exocyst genes which is strongly expressed only in stigma and not in the pollen or style. Suppose that we have an RNA knockout gene construct as...

01 January 2016 8,634 0 View

Can you please list some ways of creating variations in plants?

What are the different methods do you know so as to create genetic variations in plants?

12 December 2015 5,482 3 View

Can we say that the gene is probably regulated by the sugars if their promoters contain conserved cis-elements like other sugar-regulated genes?

Sugars regulate gene transcription by modulating promoter activities. Conserved cis-elements have been identified in the promoters of many sugar-regulated genes -- SURE motif (sugar-responsive...

12 December 2015 5,813 4 View

Should I go for sequencing?

I transformed XL1B cells by cloning 16S rDNA gene of a thermophile strain to pTZ57R/T vector. The cloned plasmid vector isolated from white recombinant colonies were quite good in the gel, and the...

11 November 2015 2,487 5 View

Growing Agrobacterium at 28-30 in liquid culture overnight

Many papers and standard lab protocols describe growing Agrobacterium at 28 degrees. However, few papers also describe doing the same at 29 degrees, and some protocols give a freedom of 28-30...

01 January 1970 6,510 2 View