Can we readily expect homozygous knockouts in the T0 plants in CRISPR knockout event of tomato plants?

Can we readily expect homozygous knockouts in the T0 plants in CRISPR knockout event of tomato plants? Or should we wait until the T1 plants after the selfing event?

08 September 2017 4,339 4 View

Are there any commercial CRISPR/Cas9 vectors suitable for potato transformation via Agrobacterium?

04 May 2016 2,927 3 View

After gene knockout, will incorporation of non-target tissue mRNA make significant error in measuring relative expression level against the wild type?

An SLR1 promoter is a tissue-specific promoter for exocyst genes which is strongly expressed only in stigma and not in the pollen or style. Suppose that we have an RNA knockout gene construct as...

31 December 2015 8,536 0 View

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies?

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies? Or are both of these TALEN pairs designed to be...

31 December 2015 258 2 View

Do tetraploid/ tetrasomic potatoes suffer from inbreeding depression as frequently as do the diploids during the conventional plant breeding?

31 December 2015 1,645 1 View

Can we say that the gene is probably regulated by the sugars if their promoters contain conserved cis-elements like other sugar-regulated genes?

11 December 2015 5,733 4 View

Should I go for sequencing?

I transformed XL1B cells by cloning 16S rDNA gene of a thermophile strain to pTZ57R/T vector. The cloned plasmid vector isolated from white recombinant colonies were quite good in the gel, and the...

10 November 2015 2,418 5 View

Growing Agrobacterium at 28-30 in liquid culture overnight

Many papers and standard lab protocols describe growing Agrobacterium at 28 degrees. However, few papers also describe doing the same at 29 degrees, and some protocols give a freedom of 28-30...

01 January 1970 6,294 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View