Thanks Dr Lee!!!

Pooling biological replicates gains you no statistical advantage in a quantitative experimental design. Pooling six biological replicates into 1 sample is statistically equivalent to using 1 biological replicate to start with. This is because you have no way of knowing the quantitative distribution within your pooled sample (i.e. is it one outlying replicate that is driving your total quantity in the pooled sample or are all the replicates contributing similarly? without getting replicate quantities individually you would never know). I have seen a disturbing trend in the past few years with researchers trying to do this in qPCR and RNA-seq experiments. It is rarely if ever appropriate and at least 2 papers I have reviewed in the last 6 months have been rejected because of it. Having appropriate controls and providing adequate biological replication are the two cornerstones for advancing our scientific understanding. There are no shortcuts for this unfortunately.