What type of interaction exist between polymer and nanoparticle ?

PMAO amhiphilic polymer was coated on the surface of NaYF4 nanoparticles.What type of interaction exist between the polymer and nanoparticle surface?

13 February 2019 6,104 6 View

Ways to prepare biological samples for HPLC and LCMS?

Hello All, I will soon start to use HPLC and LCMS for 2 different studies. I have an animal tissue and a cell culture to be used for both the studies. The question that I have is what is the...

31 August 2017 9,027 3 View

What are the different types of cells in HNSCC tumor samples?

Could anybody please tell me what are the different types of cells that originate from HNSCC patient samples. What are the type of studies that it can be used for? I understand there are...

25 August 2016 311 2 View

How does an intron spanning and exon-exon primer assay work to avoid gDNA contamination?

I am currently working on an RT-PCR experiment designing different set of genes for running on a 96 well plate. When contacted several companies, some said they adopt intron spanning primer assay...

28 January 2016 3,702 2 View

How do you choose the right cell line for breast cancer research?

01 May 2015 4,758 1 View

Can we use a lentiviral (promoter/ expression) construct for transfection in MCF7 cells by lipofectamine based method?

Can we use a lentiviral (promoter/ expression) construct for transfection in MCF7 cells by lipofectamine based method? If not how can I modify the construct for an antibiotic based selection...

10 April 2015 1,692 10 View

Student Feedback- Perception

What are the general perceptions that students have with regards to providing feedback? Are there hindrances that prevent students from being honest and constructive in their opinions? Existence...

01 January 1970 9,032 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

What is the detection limit of ED-XRF technique for transition metals in silicon ?

Hi everybody, please, can someone tell us if the transition metals (W, Fe, Ni, etc ...) in silicon destined for solar cell manufacturing can be detected by ED-XRF technique. Note that the...

18 February 2021 6,231 4 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View

How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View