There are enzymes used for dissociation of cells. Gibco has variety of trypsin. it had .25% trypsin and trypsin with different con of EDTA. My query is when do we use trypsin and when to use trypsin EDTA. does this effect the passaging of the cells (problem in attachmnet or more dead cells after trypsinisation)
Appreciate your inputs on the same.
Thanks
S. Béatrice Marianne Ewalds-Kvist
https://www.thermofisher.com/fi/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/trypsin.html#
check this out
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