I have brain homogenates (supernatant after centrifugation) stored in -80 long ago. I need to do genotyping in those samples but i do not have blood of those samples. What i have is homogenates prepared for western blotting and pellets remaining after taking supernatants. I am just curious to know if i can use homogenates to isolate genomic DNA and do genotyping.
Hi Nirmal. Well, it is fortunate you stored them in -80. I would be very surprised if you were not able to isolate a reasonable amount of gDNA from them, of good enough quality for genotyping. However, because it sounds like it was homogenized in the absence of a protein denaturing reagents, the DNA is likely to be sheared. Just make sure you get a protein denaturing/inactivating reagent onto them at the time of thawing, so the DNAses don't have any chance of working.
Best regards
Roger Latham
Thanks for information. I also have pellets. can i isolate DNA from Pellets ?
Yes I expect you could, though it will be loaded with a lot more protein and fat, which can make for troublesome extraction.
- Badges
- Science method