Hello,
I want to know if the CT26 cell line issue from Balb/c mice can induce colon cancer in an other strain like Swiss mice. Or, to induce a cancer, the cell line must be from the same strain?
Thanks.
Hello, I am working to generate colorectal cancer from colitis in mice, so I use DSS (Dextran Sulfate Sodium) in drinking water. In my first experiment I used a low molecular weight of DSS < 40...
01 February 2018 6,300 0 View
After the RNA isolation from fresh colorectal cancer biopsies, I dose my RNA's concentration, and I find about 3000µg/ml. In RT, I take 5µl of my RNA at 1ug : Ci= 3000 µg/ml Cf= 200 µg/ml...
01 February 2018 3,200 5 View
Hello everyone, I need to know if the MDSCs cells can be isolated by magnetic beads, without using the flow cytometry to sort them, I found in an article a comparison between MACS and FACS...
04 May 2016 1,535 7 View
Hello, Please I need to know how to calculate 4% of DSS ? I found in a protocol, that we need 4g ---> 100ml of autoclaved water, and to allow 5ml of this solution for each mice. I found that...
10 November 2015 2,878 2 View
Hello, Please i need to know how we can calculate 7,5mg of AOM / Kg of mice body weight. I found in a protocol of nature that we must inject 100ul of the working solution diluated 1:10 from the...
10 November 2015 4,694 2 View
Hello, I would like to know how to perform the hemoccult test in mice, knowing that we have 5 mice per cage, and how to distinguish between those with Positif hemoccult and those negatif.
01 February 2015 1,910 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View