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Questions related from Asmaa Merzouk
After the RNA isolation from fresh colorectal cancer biopsies, I dose my RNA's concentration, and I find about 3000µg/ml. In RT, I take 5µl of my RNA at 1ug : Ci= 3000 µg/ml Cf= 200 µg/ml...
02 February 2018 3,169 5 View
Hello, I am working to generate colorectal cancer from colitis in mice, so I use DSS (Dextran Sulfate Sodium) in drinking water. In my first experiment I used a low molecular weight of DSS < 40...
02 February 2018 6,280 0 View
Hello everyone, I need to know if the MDSCs cells can be isolated by magnetic beads, without using the flow cytometry to sort them, I found in an article a comparison between MACS and FACS...
05 May 2016 1,503 7 View
Hello, Please i need to know how we can calculate 7,5mg of AOM / Kg of mice body weight. I found in a protocol of nature that we must inject 100ul of the working solution diluated 1:10 from the...
11 November 2015 4,677 2 View
Hello, Please I need to know how to calculate 4% of DSS ? I found in a protocol, that we need 4g ---> 100ml of autoclaved water, and to allow 5ml of this solution for each mice. I found that...
11 November 2015 2,850 2 View
Hello, I want to know if the CT26 cell line issue from Balb/c mice can induce colon cancer in an other strain like Swiss mice. Or, to induce a cancer, the cell line must be from the same strain?...
10 October 2015 1,110 0 View
Hello, I would like to know how to perform the hemoccult test in mice, knowing that we have 5 mice per cage, and how to distinguish between those with Positif hemoccult and those negatif.
02 February 2015 1,887 4 View
