While working with PTC sometimes we encounter rooting first in the explant and so can we develop protocol having the root initiation first.
If you look at these roots from callus: they are white and thin and do not resemble real roots.
Even though lateron you get rooted shoots, after changing medium, one cannot be sure if these are funcitonal roots and well connected to the shoot whithout breaking off. Its a tricky business.
Hi, Gaurab,
General rule that first must be shoots and thereafter roots. Roots signal coming form shoots. If you get rooting form nor shoot signal, you can not get shoots thereafter.
If you look at these roots from callus: they are white and thin and do not resemble real roots.
Even though lateron you get rooted shoots, after changing medium, one cannot be sure if these are funcitonal roots and well connected to the shoot whithout breaking off. Its a tricky business.
Yes, In invitro, The first process of any plant species is to initiate shoots and then the roots. Thats how the method is standardized as part of natural process of dominance of shoots over roots in growth. Your question is good but answers may be still elusive to find.
From my experience with tissue cultures of bulbous and herbaceous plants, the roots would appear first on explants which are not cut very well off the remaining root tissue from the original plant - this is the case at the stage of initiation of cultures. If there was rooting in any of the next transfers on fresh medium, this would be simultaneously with the development of shoots.
Hi
Usually shoots appear then, roots be generated on it. If roots appear first, shoots couldn't generation on it because there are no buds, on roots, can develop to form shoot. SO, root formation is preferable only when there are secondary metabolites produced from roots.
yes, Sometimes from callus which is undifferentiated mass of cells may develop root and at the same time from other region of the same callus it may develop shoot/ small bud. In this case the root is not real/ true root. The true root should develop from pericycle which is part of shoot. This is the reason why we prefer shoot induction first and then root.
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