Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

27 November 2020 7,540 2 View

What are the alternative way to identify the stem cells present in cord blood??

commonly used practices for the identification and characterization of the kind of cell in cord blood other than Flowcytometry and reverse-transcription polymerase chain reaction (RT-PCR).

08 August 2020 6,881 3 View

What is the difference between Hex Dominant and Cut Cell Meshing methods in CFD?

Though both the methods look similar I believe there may be differences in them. What are the disadvantages of cut-cell meshing method and why is not widely used in research in comparison with...

12 July 2020 5,636 3 View

Does anyone have GS-990 DIW EIW water filtration equipment from Advantec Japan?

I tried to contact [email protected] but no response so kindly help me in this regard. We have an Advantec GS-990, it was purchased a long time ago from a Korean Collaboration. Currently,...

16 June 2020 9,890 1 View

What is the difference between anionic and cationic redox probes?

I have done Kinetics study of two different probes namely Fe(CN)63-/4- and Ru(boy)2+ on glassy carbon electrode. I kept the potential between -0.2 to 0.6 and 0.8 to 1.2 respectively. In FeCN when...

25 April 2020 3,364 10 View

Is SIMSCALE software a good to go option for visualizing and understanding academic concepts?

On what scale can SIMSCALE simulation software be helpful for students in their engineering course work comprising CFD ( Computational fluid dynamics ), thermal and structural concepts? Can...

29 March 2020 2,952 3 View

Is the Reference Intensity Ratio of Martensite(7.98) and that of Austenite (10.81), standardized against corundum, constant?

I came across the RIR value of Martensite in a paper, which is 7.98. Similarly for Austenite, which is 10.81. I want to quantify Retained Austenite in my heat treated samples. I am using Reference...

26 February 2020 9,401 2 View

Can a thermoelectric material may have negative lattice thermal conductivity?

I have calculated electronic components of thermal conductivity by wiedmanfranz law. Then the lattice thermal conductivity is derived from it. But some of those values are negative. at the end of...

26 January 2020 1,507 4 View

Anyone familiar with protein family with highly conserved C-terminal BUT very DIVERSE N- terminal, within same protein family?

Hi friends, I have been analyzing a protein family, called Fibrinogen domain related proteins, commonly called FReD or FREPs. I confronted with problem that C terminal part of this family proteins...

08 January 2020 1,320 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View