I have AFM data having images in micro metre size but i want to calculate the average size of particles and their size distribution....please suggest me what to do?
Bushra Afzal I assume you're serious. There are 1000 nanometers in a micrometer... As Drexler said “The micron scale is volumetrically 109 times larger than the nanometer scale. Confusing microtechnology with molecular technology is like confusing an elephant with a ladybug”
Transfer your micrometers to nanometers. However, check your images and pixel scales. As previously suggested, I would use another technique to measure nanoparticles, such as TEM.
Alberto Baldelli
Sir, which sofware i should use for that? And i want to calculate the size distribution of nanoparticles...how to do that?
Use wsxm or gwyddion software for this. You can use imageJ software.
http://gwyddion.net/
http://www.wsxm.es/download.html
https://imagej.nih.gov/ij/download.html
Dear Bushra, try ImageJ, it is free and it does a lot of image processing.
Otherwise, if you have Matlab, there are some ready packages to use.
Basanta Kumar Parida thanks a lot
Alberto Baldelli okay i have Image J and Gwyddion...i ll try them.
Hi.
I strongly suggest using WSxM software, it is free:
http://www.wsxm.es/download.html Then, you can do Process->Flooding to isolate the nanoparticles from the background and calculate the height histogram. *Please remember than in AFM you always have the convolution with the tip, so the lateral size of the nanoparticles in the AFM images is always bigger than the actual size. Thus, the only value you can trust to obtain the size is the height. *In the attached file you can find an example. *If you like this answer, please click Recommend.
Jose-Miguel Garcia-Martin
Thank you so much for your kind attitude...i ll see it definitely...
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