Hello, I had a question about GVHD. We wanted to investigate the effect of a cytokine in GVHD, but we don't have the possibility to use a GVHD mouse model. Can we do it by using stimulated PBMCs and doing co-culture on cell lines?
Thank you for guiding me.
Gary Lee Gilmore
I think what you are asking is can you take PBMC from two different individuals and incubate them together as a in vitro model for graft versus host disease? Yes. That is known as a MLR - mixed lymphocyte reaction. Typically, one set of PBMC is inactivated by irradiation or mitomycin C treatment first [this is called a one-way MLR]. You can use this to test the effect of adding compounds into the MLR to see if it affects the response.
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