Dear Sneha Bansode,

please check, if the attched paper might help you.

Frank

Understanding Binding Affinity: A Combined Isothermal

Titration Calorimetry/Molecular Dynamics Study of the

Binding of a Series of Hydrophobically Modified

Benzamidinium Chloride Inhibitors to Trypsin

† ‡ ‡ ,†

Reinskje Talhout, Alessandra Villa, Alan E. Mark, and Jan B. F. N. Engberts*

Contribution from the Physical Organic Chemistry Unit, Stratingh Institute, UniVersity of

Groningen, and the Groningen Biomolecular Sciences and Biotechnology Institute,

Department of Biophysical Chemistry, UniVersity of Groningen,

Nijenborgh 4, 9747 AG Groningen, The Netherlands

Received February 14, 2003; E-mail: [email protected]