The one blast search of my sequence result hit putative glutamine synthase. Nomenculature distinguishes the enzymes synthase and synthetase, but is it possible these enzymes are same?
http://www.uniprot.org/uniprot/Q8RW39
https://www.ncbi.nlm.nih.gov/nuccore/AJ277561.1
Well. I guess they are different because Synthase are enzymes that catalyze the synthesis of a biological compound but do not use ATP as a source of energy.
but Synthetase uses ATP as the energy source.
As far as I can tell, there is only one enzyme, glutamine synthetase, or glutamate-ammonia ligase (EC 6.3.1.2), which catalyzes this reaction:
ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
People were not always consistent in the past about the distinction between synthases and synthetases.
Hi there,
The initial distinction between synthetase and synthase is the absolute requirement of NTP hydrolysis for the activity of the former and fully dispensable for the latter. Using this definition the only enzyme able to produce glutamine from glutamate is a synthetase. However the strict definition of the 2 has been modified in the 80s as mentioned in the wikipedia page of synthase:
https://en.wikipedia.org/wiki/Synthase#cite_note-1
Which is why both naming, although confusing for purists, is allowed nowadays.
Please see the papers for your reference
It might be helpful for you
Article Characterization and improved properties of Glutamine synthe...
Article Characterization of an L-phosphinothricin resistant glutamin...
best Regards
Ashok
Hi Fatma, I would like to suggest to be carefull with annotations of genes concerning their putative function. You can be sure about the function of an enzyme, only if it has been shown experimentally and published. From my own experience, a few glutamine synthetases turned out to be glutamylating enzymes.
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