I think it is possible. Just add few Au interacting amino acid residues like cysteine on the N or C terminal of GFP. I also suggest to use a monomeric GFP.

Thank you, Can you please forward any papers if you have regarding GFP tagging.

Yes, you can. You need to get a carboxylated gold and use EDC coupling chemistry to attache the amine residue from GFP to the AuNP. Carboxylated gold NPs are available at Ocean Nanotech. You can use their conjugation kit for this purpose.

Yes Aishwarya, you can. If you need any information about that, I will send you a file regarding the related procedure.

Yes, it is possible to electrostatically assemble negatively charged fluorescent proteins/polymers onto cationic gold nanoparticles or the positively charged fluorescent polymers/proteins on anionic gold NPs. This would result in the quenching of fluorescence and can be used for the detection of other proteins, pathogenic bacteria and even cancerous tissues/cells. Professor Vincent Rotello at UMass, Amherst, has done a lot in this regard and you may visit his page 9https://sites.google.com/site/rotellogroup/) for further information. May search for his articles on Chemical Nose. All the best.....

I think its possible. I think as Zoraida Aguilar said its possible and specialy cysteine is the most important AA to do this work. One of my senior tried that several times but it didnt work. I am sure it depends with nanoparicle size.

Yes you can. However, the fluorescence will get quenched by gold unless you maintain proper chain lengh of the linked. Most of the time it you cannot get rid of the quenching.

Thank you very much all, If you have any material regarding GFP tagging please forward it to me, it would be helpful. Thanks again.

I think we have few reports of binding flourescent proteins on the modified surface of the gold naoparticles. For ur conveninece I am attaching here few reports

Please find one more report for of your intrest.

Thank you very much.