Can we compare delta Ct values of patient and control from different run with different volume of cDNA/amplification product?

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Giorgia Pertile

If you run different amount of cDNA is impossible to compare them.

Harshita Baranwal

so i have to compare Ct of patients with those controls only which were ran with same amount of cDNA?

Exactely. Because the Ct id influenced from the amount of DNA/cDNA you load inside the mix because high concentration of DNA have lower Ct than low concentration.