What could be the reason for no detection of YFP in 35S:: Gene:: YFP lines in confocal in control as well as treatment conditions?

23 September 2023 0 4K Report

Out of 10-15 seedlings only 1-2 seedlings showed YFP fluorescence. However, in RT there is 5 fold Overexpression in line 1 and 30 fold Overexpression in line 2. Even then, there is no uniform YFP fluorescence. What could be the reason?

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