Can we calculate elastic constants for doped systems with IBRION=6 in VASP ?
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
I am writing a paper as a part of my course. I am new in London and was wondering that what publications should look upto?
21 July 2024 3,538 1 View
After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...
18 July 2024 4,674 4 View
It has been a quite long time since the Russia and Ukraine war has been going on and if we see the context of Geopolitics and International Relations we don't see a mediation between the two...
18 July 2024 6,737 4 View
One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...
17 July 2024 9,668 4 View
Please help me in plotting the photon-magnon coupling plot as shown below
14 July 2024 1,632 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I heard that as the value of "num_iter(tag in wannier 90)" is higher, spread of Wannier function(=WF) is gradually lower in wannier 90. If so, is this procedure that minimize the spread of WF...
13 July 2024 4,608 0 View
I am having trouble expressing a protein and have heard that co-transforming it can improve stability and increase expression, so I am looking to try this experiment. I only need the kinase domain...
08 July 2024 2,935 5 View
How to store SDS-PAGE for fluorescent imaging without fixatives? I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of...
29 June 2024 8,720 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
To calculate the spin density using Gaussian software, is it necessary to provide the IOP tag? If so, what is the significance of this requirement?
02 June 2024 4,001 1 View
I am expressing an mCherry tagged protein. Yield of total protein is good but the fluorescence intensity is very low (ie. Protein assays and gels show a lot of protein but this does not correspond...
28 May 2024 4,828 1 View
I am preparing a case study of a social work professional organisation which is non-existent now but had a glorious past. For this, I interviewed around 20 members of the association to understand...
18 May 2024 6,656 3 View
I wanted to express protein in mammalian cells for purification I need 6XHis tag, however, plasmid synthesis company have vector with Myc and his tag. So do I need both or only his tag.
12 May 2024 941 4 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? Targeted gene part is 18bp long But...
10 May 2024 3,106 4 View