Every articles that i have studied for assessment of ld50 by up and down procedure have used oral route for animal treatment. I wanna know if it possible to use I.P route for assessment of ld50 through up and down procedure?
dear Abolfadhl
of course you can do it but be carful of the dose and the waiting time >2 days
http://www.ncbi.nlm.nih.gov/pubmed/3987991
have a nice day
Yes, and others too (IM, PO) always this depend of what you think evaluate, some pharmaceutical product or microorganisms for example.
Hi! Maybe the question is: what is the usefulness of this test? For pharmaceutical products, in 2015 it is no longer needed to estimate the LD50, even no longer needed to conduct acute toxicity assays (provided that other more relevant assays are conducted). For cosmetics, no longer permitted to use animals. For chemicals or biocides it is still required to have a rough estimate of lethal dose (not LD50), but the data should be relevant to assess the risks. So, it make sense to perform the studies by a route that predict the actual risk of humans or animals exposed to the product. It is possible to use the i.p. route, but I wonder if the results from i.p. route can predict easily eventual risks of intended/accidental human exposure, or if the doses used can be easily extrapolated to others more relevant to actual exposure. Certainly, the acute toxicity assays may have several purposes, and a key point is to wonder yourself if the study design is relevant for the risk to asses.
Hi cunchillos,
My LD 50 studies were done by oral route, but now I want to assess the activity by giving the ip.. !
Is that necessary, that if we conduct the LD50 in oral route and the treatment should also be done in the same route (Cant we change the route of administration)
Some of chemicals may not show toxicity in oral route, but they might be toxic with the other routes or vice versa .In such case, how can we fix for route of administration.
Why most of the people suggesting to go for oral route?
The reason you use the oral route is to maximize the effect of first pass metabolism which often leads to the formation of toxic metabolites. These metabolites may still be formed but to a differential extent after other routes of administration such as IV or IP. This is particularly important if the parent molecule is nontoxic but a metabolite is.
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