I will have to extract total DNA, meaning nuclear and mitochondrial DNA from urine samples. Since it will take weeks for the sampling, I need to store them before using them. How to store these samples for 2 weeks or 10 days before the lab extraction and processing?
Urine can be stored at room temperature for more than 2 years using Norgen Biotek Urine Preservative. Spinning down the cells and storing at -80C is a good idea but in this case you will be only isolating DNA from exfoliated cells only. You can isolate total DNA from urine (i.e. cellular and free-circulating DNA/trans Renal DNA) from urine using one of Norgen's Urine DNA Isolation kit (slurry Format) that can isolate DNA from up to 25mL of urine.
I am not familiar with Urine samples since I used mainly blood samples, but you can try to spin down the samples to collect cells, store them at -80 deg for further analysis including the DNA extraction.
Urine can be stored at room temperature for more than 2 years using Norgen Biotek Urine Preservative. Spinning down the cells and storing at -80C is a good idea but in this case you will be only isolating DNA from exfoliated cells only. You can isolate total DNA from urine (i.e. cellular and free-circulating DNA/trans Renal DNA) from urine using one of Norgen's Urine DNA Isolation kit (slurry Format) that can isolate DNA from up to 25mL of urine.
Dear Moemen
Do u know what s the best way to keep urine samples for further RNA extraction?
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