Did you find an answer to this. I'm trying with IFN-Y + LPS and IL-4 + IL-13 at 50ng/ml. I'm hoping to demonstrate states using FACS but immunocytochemistry might be an option.
no i havent find any answer. at what conc. you are using the cytokines and LPS and for what period of time of incubation and what markers you are looking for. once i tried for iNOS and arginase marker but didnt get proper results .