H3K27me3 (17kDa) western blot not working - help?

I'm using SDS-PAGE to try to detect histone 3 lysine 27 trimethylation (H3K27me3) using a ChIP grade monoclonal antibody from Diagenode. I'm loading 15ug of protein from U937 nuclear extracts, a...

12 December 2016 700 4 View