Can poly ethylene glycol diacrylate (PEGDA) hydrogel microparticles have negative zeta potential value?

Hello, I measured the zeta potential of PEGDA hydrogel microparticles which is a cube with a side length is 20 um. Particles are suspended on DI water + tween 20 Since particles are only composed...

25 October 2020 520 3 View

Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

A vertical line at the bottom of my SDS-PAGE gel?

Dear, Whenever I run an SDS-PAGE, I get an unwanted vertical line appearing at the bottom of the gel. Does anyone have any idea why this happens? Any comments or suggestions would be highly...

10 February 2020 8,509 1 View

How to quantitatively determine the pyridine in water?

I want to find the amount of pyridine in water after passing through a membrane. We usually deal with concentrated pyridine solutions and it would require a large amount of water for diluting the...

31 October 2019 9,251 3 View

What if the primer becomes shorter in PCR?

11 February 2018 534 4 View

What is the mechanism of Metal Fluorescence Enhancement?

Hello, I'm currently interested in metal fluorescence enhancement. As far as I know, metal can enhance the intensity of fluorophore. Is it able to use any kinds of fluorophore without its...

14 June 2017 5,498 2 View

Is it able to use FRET(fluorescence resonance energy transfer) as a fluorescence amplification method?

Hello It is well know that FRET can be used to measure the interaction between two molecules. I wonder if FRET can be used to amplify fluorescence signal by placing numerous acceptor near each...

11 June 2017 381 4 View

What is the relation between assay temperature and miRNA specificity?

Hello, I'm working on detecting miRNAs using hydrogel microparticles with probes incorporated in it. Recently, I found an article about the relation between melting temperature and specificiy of...

28 July 2015 6,031 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View

What is the best antimicrobial agent among metal oxides?

I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?

21 January 2021 7,068 5 View