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Questions related from Yoonho Roh
Hello, I measured the zeta potential of PEGDA hydrogel microparticles which is a cube with a side length is 20 um. Particles are suspended on DI water + tween 20 Since particles are only composed...
26 October 2020 663 3 View
Hello, I have a question about primer length. What if the primer becomes shorter about 8~10 mer and set annealing temperature between 25~30 ℃ ? Will there be problem in proceeding PCR? Thank you.
12 February 2018 584 4 View
Hello, I have a question about how association constant changes according to the probe density and salt concentration in nucleic acid hybridization. I knew from experiment that high probe...
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Hello, I'm currently interested in metal fluorescence enhancement. As far as I know, metal can enhance the intensity of fluorophore. Is it able to use any kinds of fluorophore without its...
15 June 2017 5,543 2 View
Hello It is well know that FRET can be used to measure the interaction between two molecules. I wonder if FRET can be used to amplify fluorescence signal by placing numerous acceptor near each...
12 June 2017 430 4 View
Hello, I'm trying to reduce thiol group (S=S) linked with DNA. To prevent degradation, thiol group linked with DNA is stored in 1x Tris-EDTA. For the reduction of thiol group, TCEP...
28 February 2017 9,207 2 View
Hello, I'm working on detecting miRNAs using hydrogel microparticles with probes incorporated in it. Recently, I found an article about the relation between melting temperature and specificiy of...
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