Can total proteins be measured by Bradford assay after homogenizing the sample with sucrose buffer (0.25M)?

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Adam B Shapiro

Sucrose does not interfere with the Bradford assay.

Marcelo N. Avelar

Yes, small amounts of sucrose can interfere with the analysis. You can coprecipitate the protein with calcium phosphate and wash it before adding the reagent with 80% ethanol.

Mohan Kumar S

No interference of sucrose