Dawid Skoczek

3 Questions 3 Answers 0 Followers

Questions related from Dawid Skoczek

Can the RIPA buffer be supplemented with DNAse?

Hi everyone! I have a problem with slime in my protein sample. For isolation, I use RIPA buffer + protease inhibitors. When I measured the protein concentration immediately after isolation...

07 February 2024 4,774 1 View

How can I show RT-qPCR data of two different cell lines?

Dear All, I would like to ask how I should visualize the mRNA expression of a single gene (HNF1A) on a graph. I have my positive control line (HepG2), in which the expression of the gene of...

28 October 2022 7,833 6 View

Problems with Comet Assay (lysis or electrophoresis).

Dear Colleagues, I'm struggling with the comet assay - I can't see the expected comet shape. I work with three isogenic lines, including the control line, monoallelic, and biallelic mutant. I do...

01 January 1970 6,455 0 View