How to Detect Water in THF (Tetrahydrofuran) ?

Hello, we have solvent purifier system in our lab. But There is some unwanted results for our experiment. We are suspecting purity of solvents. Are there any simple methods to detect water in THF?

19 November 2020 114 5 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

03 March 2020 8,177 4 View

How does the amount of amino acid exceed the amount of crude protein in fish meal (Dry matter basis)?

I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...

16 July 2019 2,039 6 View

Which one should I choose from the results of a sample with different amino acid analysis methods?

I have a protein source with varying results of amino acid profile. For instance; when the sample is hydrolysed with "6 M HCI" L-Valine level is 0.24g/100g whereas it is 3.08g/100g when...

23 February 2018 5,648 2 View

What is the difference between higher order (second order) confirmatory factor analysis and bifactor analysis?

24 July 2017 5,178 5 View

How to determine total RNA concentration when you have large amount of miRNA?

I am isolating total RNA with miRNeasy micro (PBMCs) miRNeasy serum plasma (serum) and exoRNeasy (serum/exosome) for Next Gen Sequencing for miRNA. I believe my final product should contain mostly...

30 March 2016 3,006 4 View

How can I do a reaction between polyamine and lipoic acid NHS ester in an organic solvent?

I want to do this reaction but as far as learned from literature, NHS ester-amine reaction is done in a phosphate buffer solution between pH 8 to 9. However, lipoic acid modified polymer...

27 June 2015 1,731 3 View

An economic method for the fluorescent labeling of PCR fragments?

I have a trouble with my pcr reactions and i want to ask you where is my fault.I designed six primer pairs according to Schuelke's M13 methods but I did not get any reaction.One of my primers...

23 December 2014 4,623 5 View

What is the reason for different fold changes in gene expression from independent experimental repeats in Real-time PCR experiments ?

25 October 2014 7,228 3 View

How should I calculate a touchdown PCR reaction with a low dna quantity?

14 September 2014 2,091 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View