Hi I have prepared my samples for western blot but won't be able to run them except after 2 weeks can I store them in -20 after adding SDS and boiling them or will they be ruined?
Thanks in advance
Maria Attallah, In our laboratory after sample preparation for western blot, we have stored in -30 for more than 6 months. I think 2 weeks is not a big problem at all. Hope for the best.
Maria Attallah, In our laboratory after sample preparation for western blot, we have stored in -30 for more than 6 months. I think 2 weeks is not a big problem at all. Hope for the best.
In my experience, there is no problem. I also have kept them -20/ 2-3 weeks.
I have used my sample after ~1 year (Stored at -20 or -80), and everything was fine.
It's dependent on which types of samples you are using. Almost all samples you can keep them on -20 (short time storage) or -80 (long time storage) after boiling with SDS. Except, if those samples are membrane proteins or if you want to check some post-translational modification (e.g: phosphorylation).
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