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Questions related from Maria Attallah
I have done dialysis after eluting my protein with 300 mM imidazole. My dialysis solution was10% glycerol in PBS pH 7.4 I found my solution a bit cloudy which indicates I have some aggregation...
14 May 2021 7,777 4 View
is there any trouble that would result from incubating Quick CIP for 1 hour. NEB recommendation is to do it for 10 min only, isn't that a bit too short or has anyone tried it and it works just...
11 March 2021 738 6 View
I am planning my animal study and would appreciate it if someone can provide advice regarding this Is it possible to use 20 mM HEPES buffer to dissolve my treatment for in vivo study for...
28 September 2020 5,586 3 View
Hi I have prepared my samples for western blot but won't be able to run them except after 2 weeks can I store them in -20 after adding SDS and boiling them or will they be ruined? Thanks in advance
22 April 2019 7,746 7 View
Recently I have been working with NTC nanoplasmid, has anyone used them? They state in their manual that after transformation of bacteria, it should be grown at 30 degree, how long does the...
15 March 2019 6,694 0 View
i have spreaded 100 mcL of my culture on the agar plate and it has been 45 min and still not absorbed how long does it usually take to dry? many thanks
26 February 2019 8,864 2 View
this is my first time doing siRNA transfection I just would like to know if I am seeding a 6 well plate with cells do I use complete media or optiMEM? Also upon treatment of the cells do I add...
30 January 2019 7,338 8 View
If I am trying to develop a DNA vaccine what are the most preferable vectors to use?
26 November 2018 8,939 5 View
