Maria Attallah

7 Questions 10 Answers 0 Followers

Questions related from Maria Attallah

Has anyone used Quick CIP and incubated it for 1 hour at 37C?

is there any trouble that would result from incubating Quick CIP for 1 hour. NEB recommendation is to do it for 10 min only, isn't that a bit too short or has anyone tried it and it works just...

11 March 2021 716 6 View

Is it possible to use 20 mM HEPES buffer to dissolve my treatment?

I am planning my animal study and would appreciate it if someone can provide advice regarding this Is it possible to use 20 mM HEPES buffer to dissolve my treatment for in vivo study for...

28 September 2020 5,554 3 View

Can the boiled samples for western blot be stored for 2 weeks at -20 or not?

Hi I have prepared my samples for western blot but won't be able to run them except after 2 weeks can I store them in -20 after adding SDS and boiling them or will they be ruined? Thanks in advance

22 April 2019 7,721 7 View

Has anyone used NTC nanoplasmid?

Recently I have been working with NTC nanoplasmid, has anyone used them? They state in their manual that after transformation of bacteria, it should be grown at 30 degree, how long does the...

15 March 2019 6,669 0 View

How long does it take for the culture to be absorbed by the agar after spreading?

i have spreaded 100 mcL of my culture on the agar plate and it has been 45 min and still not absorbed how long does it usually take to dry? many thanks

26 February 2019 8,846 2 View

SiRNA transfection using lipofectamine RNA imax?

this is my first time doing siRNA transfection I just would like to know if I am seeding a 6 well plate with cells do I use complete media or optiMEM? Also upon treatment of the cells do I add...

30 January 2019 7,318 8 View

What are the best vectors to be used for DNA vaccines?

If I am trying to develop a DNA vaccine what are the most preferable vectors to use?

26 November 2018 8,907 5 View