Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Train-test split numbers for YoloV3/V4 models?

Dear Research Community, What are the common numbers (in percentages) for splitting the image dataset for training a Yolov3/V4 models? Is there a formal research on this question? Azmyin

08 February 2021 647 3 View

How are the anchor sizes defined in YOLOv3 and YOLOv4?

Dear research community? How are the anchor sizes defined in the cfg files of YOLOv3 and YOLOv4 object detectors? For example from the Yolov4 cfg file...

22 January 2021 4,504 1 View

What are core advantages of 2nd & 3rd generation panel data techniques over the 1st generation techniques?

I am interested to know about the difference between 1st and 2nd and 3rd generation panel data techniques.....

31 December 2020 7,204 7 View

How to construct a combined contour plot of two different text files in python?

As I constructed sperate contour plot for each methane and ethanol but now I wanna draw a combined plot for methane/ethanol ratio (for selectivity purpose)?

30 December 2020 4,531 1 View

Technically is there any difference between policy implications and policy recommendations?

Usually we use the two terms interchangeably in our articles but whether is there any technical difference between them?

26 December 2020 185 18 View

Modeling several ODE in matlab to create a system?

Please I have 4 groups of 8 sets of differential equations that I want to use for simulations. Each set has 8 differential equations to form a group. Each group is coupled with another group...

14 December 2020 8,193 10 View

What are the tools and techniques for making Custom dataset for YOLO?

Dear Research Community. I am new to the field of deep learning based object detection techniques. At this time, I am working on a project which will not use front-facing camera to detect people....

13 December 2020 957 3 View

What devices can an independent researcher use to study plant electrophysical signals, plant bioacoustics, Action Potential, Variation Potential etc?

I have heard of the Plant Spikerbox from Backyard Brains and trying to acquire it. I want to study the electrophysical changes in common plants as Chilli, Tamarind, Papaya, etc due to...

04 December 2020 6,308 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can the intensity of this broad band in XRD mask other crystaline phases present?

I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....

19 February 2021 8,414 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View